The treble staff is the upper of the two staves in the grand staff. In addition to its position, it is also designated by the treble clef, which is placed at the very left in each set of the five lines of this staff on sheet music. A note placed on the lowest line represents the first E above middle C (which is the first ledger line below this staff), designated E4 and MIDI 64, and a note on the top line represents the second F above middle C, designated F5 and MIDI 77.